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Description

& Strohl, K

199 As previously mentioned, due to its reactivity, cysteine is not an abundant core residue in protein sequences

Cell suspension and chloroform/methanol solution (methanol: chloroform = 1: 2) were mixed with a ratio 1: 4 by volume, vortexed for 30 s and shaken for 1 h at 180 rpm at 37 C